Parkin Protein

Concentration Of Parkin Protein

Only the bottom of the preceding concentration table is visible.

row/source	visible method/text	Brain	CSF	notes
Cossu Biosource	2021? ELISA (MBS732278; MyBiosource?)			top row is cut off; visible cells include ELISA, ~60 pg/ml, ELISA, and ~150/ml
NSTM SMCxPro		0.4 pg/mg	0.06 pg/mL
DMPK LC-MS/MS		32.5 pg/mg protein
DMPK SIMOA			0.137-0.412 pg/mL

Commercial Parkin Product

product/source	WT	extra ID/catalog	full length	tagged?	activity?	denatured?	MW (kDa)	species	source	phosphorylation? / note
Ubiquigent, Cosmo bio https://www.ubiquigent.com/wp-content/uploads/2014/05/63-0048-025-30340-v2-0-0.pdf		Cat. No. 63-0048-025	o	untagged	o	?	51.6	human	e. coli	there is no mass-spec data to confirm that this protein is not phosphorylated. We have reached out to the company independently, but they have not responded to our inquiry yet At this time, I would not recommend using this protein if the phosphorylation state cannot be explicitly confirmed.
https://www.labome.com/gene/human/Parkin-protein.html										link/reference row only
H00005071-P01 Abnova https://www.abnova.com/products/products_detail.asp?catalog_id=H00005071-P01	-	-	O (1-387?)	GST			68.8			?
MyBiosource MBS1070644 https://www.mybiosource.com/recombinant-protein/e3-ubiquitin-protein-ligase-parkin-prkn/1070644			O 1-465	His-SUMO			66		e.coli	? sent email.
LS-G2736										?LS-G21035 로 보낸 이멜로 갈음될터
E3-162-025 R&D Systems	X
E3-164 boston biochem	X
ATGP2517 https://nkmaxbio.com/sub/shop.php?ptype=view&prdcode=2004013571#tab2			O 1-465	his		denatured	53.9		e.coli	? sent email. -> ATGP2517 is a denatured form so it is most likely not the best option for functional studies. It's better suited for WB or imaging assays.
ADDP follows. Parkin_protein ADDP_InStock_AsOf20211203.xlsx			?	His & GST	?					Some are yes, some are no

ADDP In-Stock Parkin Proteins

Visible Excel-like source line:

ADDP follows.
Parkin_protein ADDP_InStock_AsOf20211203.xlsx

No	Protein ID	Protein	Gene name	Source organism
1	TBVC-10241_1A	His-Avi90-GST-hPARK2v1	PARK2; PRKN	Human
2	TBVC-10241_2C	His-Avi90-GST-hPARK2v1	PARK2; PRKN	Human
3	TBVC-10241_3E	His-Avi0-GST-hPARK2v1	PARK2; PRKN	Human
4	TBVC-10241_3F	His-Avi0-GST-hPARK2v1, phospho	PARK2; PRKN	Human
5	TBVC-10241_4G	His-Avi90-GST-hPARK2v1, phospho	PARK2; PRKN	Human
6	TECC-10187_1A	His-Avi90-GST-mPARK2vX3	PARK2; PRKN	Mouse
7	TECC-10187_1B	His-Avi90-GST-mPARK2vX3, phospho	PARK2; PRKN	Mouse

Homology Of Parkin Protein

source/person	visible statement
(Jacobo, Sarah Melissa)	Hello, I calculated here that Hs vs Mm Parkin protein is 94% identity, 98% similarity
MPK (20220728 Naohiro)	Human PRKN shares high amino acid homology with monkey (97.2%)

CLUSTAL O(1.2.4) multiple sequence alignment
Amino acid differences highlighted

Visible alignment endpoints: 60, 120, 180, 240, 300, 360, 420, 465.

KO Parkin

Visible citation/title fragment:

KO Parkin
(Pickrell, 2015 #729)

Gene activated	Species	Age of Onset	DA Neurotransmission Defects	Motor Phenotypes	SN Neuron Loss	Lewy Body Formation	References
PARK2	Human	Juvenile > 20 years Early onset 20-40 years	DA depletion in striatum	Cardinal Signs	Progressive	Absent or present	Matsumine et al. 1997 Kitada et al. 1998 Pramstaller et al. 2005
PARK2	Drosophila	N/A	Absent	Flight wing degeneration, motor coordination deficits	Absent	Unknown	Greene et al. 2003
PARK2	Drosophila	N/A	Absent	Flight wing degeneration, motor coordination deficits	Absent	Unknown	Pesah et al. 2004
PARK2	Drosophila	1 day (DA neuron loss progressive)	Unknown	Flight wing degeneration, motor coordination deficits	DL region	Unknown	Whitworth et al. 2005
PARK2	Drosophila	N/A	DA depletion	Flight wing degeneration, motor coordination deficits	Absent	Unknown	Cha et al. 2005
PARK2	Drosophila	30 days	Decreased TH+ Expression	Flight wing degeneration, motor coordination deficits	DM and DL region	Unknown	Park et al. 2006
PARK2	Drosophila	25 days	DA depletion	Flight wing degeneration, motor coordination deficits	DM and DL region	Unknown	Yang et al. 2006
PARK2	Mouse	N/A	Increased extracellular DA release	Beam slip motor deficit	Absent	Unknown	Goldberg et al. 2003
PARK2	Mouse	N/A	Decreased DA uptake	Motor coordination deficits	Absent	Unknown	Itier et al. 2003
PARK2	Mouse	N/A	Absent	Startle response deficit	Absent	Unknown	Von Coelln et al. 2004
PARK2	Mouse	N/A	Absent	Absent	Absent	Unknown	Perez and Palmiter

Uncertain Spans

location	text/status	reason
concentration table	Cossu 2021?, ELISA(MBS732278; MyBiosource?), ~60 pg/ml, ~150/ml	Top row is cut by the photo.
commercial product URL	ubiquigent.com/.../63-0048-025-30340-v2-0-0.pdf	OCR sometimes reads ubiqulgent/.pdl; image appears to show ubiquigent and .pdf.
H00005071-P01 full length	O (1-387?)	Parenthetical range is small and marked with question mark in source.
KO table bottom row	Perez and Palmiter	The bottom row is visible but lower edge cuts off any possible year or continuation.