Human Biological Samples

Effective immediately, we will be using the Scientist.com platform for HBS; the ability to order HBS as catalog items in Ariba is no longer available. For definition,

Human Biological Samples include but not limited to:

• Human tissue—fresh, frozen, fixed or processed (e.g., sectioned)
• All human blood (e.g., peripheral or umbilical cord) and blood byproducts (e.g, serum, plasma, buffy coat)
• All human biofluids (e.g., sputum, urine, bile)
• Human primary cells derived from human biosamples
• DNA derived from individual donor

A company-wide HBS Policy and SOP is being created. Until then, the new process is as follows:

Scientists are responsible for:

1. Submitting requests for HBS using the Scientist.com platform. Please make every effort to work with already approved HBS suppliers there.
   • You will see the badge for approved suppliers
2. If you wish to work with Takeda- approved suppliers who are not on the platform, please let me know. They can either be onboarded or else work through the lengthier internal processes in place.
3. Selecting the vendor and requesting the SOW
4. Downloading the SOW and sending it to me

Next steps:

• I will submit a ticket and work with the CMAS team to track all activities for internal review by legal and privacy functions.
• This will include submission of an ICF template submitted by the vendor
• When all internal requirements are met, I will approve the SOW in Scientist.com.
• Once approved, requestors will be able to accept the SOW – but not before then.
• The CMAS team will use the Scientist.com PR to requisition the PO.
• Requestors and Scientist.com will be notified when the PO has been issued.

Imaging

				Example
image	Intensity (in relation to the background)	Isn't this Signal to noise ratio, Ie isn't this target-to-background ratio (eg. striatum/cerebellum)?		DatSCAN SBR in normal human (decade >50) 분당서울대 striatal BPnd in mice: mean 2.1
	Extent (indicated by shape)
찍는사람	Operator dependency
찍는 도구 (hardware, equipment)	Resolution quantifies how close lines can be to each other and still be visibly resolved.	Pixel (the smallest controllable element of a picture) count,	Spatial resolution: 기계에 쓰는 말임. in radiology refers to the ability of the imaging modality to differentiate two objects (=blurriness and sharpness, clarity). how closely lines can be resolved performance characteristic, 임. Although the clinical gamma camera can provide a tomographic resolution of about 10 mm, some preclinical SPECT scanners can provide a submillimeter spatial resolution. Image quality: Scatter correction and resolution recovery are also applied to improve resolution of SPECT images → anatomical delination Detection sensitivity	31P-MRIS: no standardized equipment (even ones) 31P-MRS: Spatial resolution does not be essentially we would be getting a signal from area of the surface coil, most typically occipital The spatial resolution of the PET images is typically ~ 4-5 mm, and the CT images is ~ 1 mm.
찍는 방법	Protocol (standardization)	Acquisition Reconstruction
사체	Inter-individual variability
ease (건강 vs 질병)	Signal window			is there a clear enough deficit that we can pick up a (partial?) reversal in a small study? Which parameter should we focus on? However those details can be addressed to some extent in preclinical studies. So I would definitely be in favor of a thorough pre-clinical evaluation of the methodology and treatment hypothesis would be necessary before considering any translational
	Spatial distribution

PET

	[11C]	[18F]	68Ga
Produced with a cyclotron?	yes shorter imaging times	Yes
half life	20.4 min,	110 min, (five times longer than 11C) → Allowing multiple patients preparaton	1 h
When no on-site cyclotron, feasible? (ie regional delivery feasible?)	Unfeasible (half life 짧아 , 운송동중에 없어짐) Dartmouth case	Feasible: 18F-labeled tracers may be produced at regional cyclotrons and delivered to imaging centers
radiation dose?	Possibly lower radiation doses.

the affinity of the radiotracer relative to the concentration of binding site present in the target tissue is the most important characteristic of site-specific radiotracers

SPO 의 Bmax 는 같으니, affinity 매우 중요하겠네. → human brain ARG (/homogenate) 에서의 Kd 값을 찾아 비교하자!

Immunity

	Line of Defense	Timeline	Cells	Antigen Dependency	Examples
(specific)	First	Immediate response (0 -96 hours)	Natural killer cells, macrophages, neutrophils, dendritic cells, mast cells, basophils, eosinophils	Independent	Skin, hair, cough, mucous membranes, phagocytes, granulocytes

Uncertain Spans

location	transcription	uncertainty
Imaging table / decade >50	the example cell reads DatSCAN SBR in normal human (decade >50); preserved verbatim.	low confidence on decade vs decade(s).
Imaging table / 분당서울대 striatal BPnd in mice: mean 2.1	reads as written; the institution prefix 분당서울대 (Bundang Seoul National University Hospital) attaches directly to the figure.	low confidence on whether 2.1 is followed by additional digits clipped at the cell edge.
Immunity table / first row label / (specific)	the leftmost cell reads (specific); the column header above reads Adaptive per side-nav statusbar bleed (Adaptive (specific)), but the visible body cell only shows the parenthetical.	low confidence on whether Adaptive was clipped.
Imaging row 1 examples / striatal BPnd in mice: mean 2.1	the trailing digit may extend (2.1...); preserved verbatim.	edge truncation.