Summary CEI tail (Anima Biotech / Hi Julia), Heatmap CEI / Landscape / Meetings CEI sharepoint links, Cell lines (Neuronal catecholaminergic / Non-catecholaminergic / Non-neuronal tables), Cerevance Datasite & Deliverables (Project Acorn / CVN424 GPR6 inverse agonist) review table

Anima Biotech - They are focused on small molecule RNA translation inhibitors. They have a tau program that is not yet partnered.

Hi Julia, Sorry I missed your message. I am available now, and any time afternoon would work for me. My number is 949 241 5449.

Heatmap CEI

				GBA
human	SH-SY5Y	adrenergic in phenotype but also express dopaminergic markers		O	Most extensively characterised in its dopaminergic system including activity of the dopamine transporter (DAT) and culture conditions (Xicoy et al. 2017, PMID).
Human	SK-N-BE
Human	BE2-M17
Rat	PC12		derived from a pheochromocytoma of the rat adrenal medulla
Rat	N27	Dopaminergic	derived from an immortalized clone of rat neurons by transfecting fetal mesencephalon cells with the plasmid vector pSV3neo carrying the LTa gene from SV40 virus [7]
Mouse	MN9D	Dopaminergi	The MN9D dopaminergic cell line was generated by fusion of rostral mesencephalic neurons from embryonic C57BL/6J (embryonic day 14) mice with N18TG2 neuroblastoma cells [10]
Mouse	Cath.a	Dopaminergi	Cath.a is derived from tyrosine hydroxylase positive tumors in the brain stem of a transgenic mouse carrying the SV40 T antigen oncogene under the transcriptional control of regulatory elements from the promoter region of the rat tyrosine hydroxylase gene [8]
Mouse	CAD	Dopaminergi	CAD cells are a variant of Cath.a cells in which the original immortalizing oncogene SV40 T antigen was lost [9].

tissue
neuronal	HEK293	human	Embryonic kidney	Immature neuronal cell type 임

Origin Tissue	Cell line	Origin species		Cell type	Phenotype
Connective tissue
Epithelium	Hep G2	human	Liver cancer
Muscle
Blood cell	RAW 264.7	mouse	leukemia	macrophage
	THP-1	Human	Leukemia (from the peripheral blood of a 1-year-old human male with acute monocytic leukemia.)	monocyte	IL-1b
	HAP1	Human	leukemia

I would everyone fill out this table to collect feedback on Cerevance’s preclinical programs.

Cerevance preclinical programs triage response 2022.xlsx

Document topics	Document link	Guidance
Data room DD Questions (due Apr 29):	Prj Acorn Questions.xlsx	Please add your questions as you review the data room to "Prj. Acorn Questions" excel
Diligence Summary (due May 3rd):	Prj Acorn Diligence Summary_TMED_Biomarker.pptx	What is your overall feedback on this opportunity? We will be evaluating Adjunctive therapy and Monotherapy separately. Please add your feedback for adjunctive therapy on slides 1-3 and for monotherapy on slides 4-6 to diligence summary deck
Friday (5/9/22)?	Cerevance preclinical programs triage response 2022.xlsx	Our request to all the functions is to continue adding your data room review feedback to the diligence summary slides □ here. Unsure at this time, but we may revisit this opportunity in future so your diligence summary is crucial for record keeping. May I kindly request of you to complete your feedback by 5/3/22?

		See
As	As adjuntive	Pramipexole etc my summary, FDA guidance, papers for unmet needs
	As monotherpy
		Tx paradigm, Unmet needs : updrs vs off-time?

target	mechanism			Design
424	GPR6 inverse agonist, small molecule, - GPR6 is an orphan GPCR that has enriched expression in the striatopallidal, indirect pathway, MSN neurons of the striatum - a target present in striatal neurons that express dopamine receptor D2. The company claims that CVN424 does not affect D1-dependent			P2	2019, a Phase 2, randomized, place-controlled study began with Parkinson's who experience motor fluctuations on a stable…

location	transcription	uncertainty
Cell lines / catecholaminergic	`Dopaminergi` (rows MN9D / Cath.a / CAD)	likely truncated `Dopaminergic` due to right-edge clipping.
Project Acorn / CVN424 cell	`2019, a Phase 2, randomized, place-controlled study began with Parkinson's who experience motor fluctuations on a stable…`	sentence clipped at right edge of photo.